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Comparing dry ashing and wet oxidation methods. The case of the rice husk ( Oryza sativa L.)
Author(s) -
Sun Xiuping,
Wu Yan,
Wang Changsui,
Hill David V.
Publication year - 2012
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22060
Subject(s) - phytolith , ashing , oryza sativa , husk , extraction (chemistry) , chemistry , botany , biology , chromatography , biochemistry , physics , pollen , quantum mechanics , gene
Experiments were conducted to determine the effects that different methods for the preparation of modern plant samples have on the resulting phytoliths using rice husks ( Oryza sp.). The methods that are commonly used in phytolith extraction include (1) dry ashing, (2) acid extraction (3) a combination of both techniques. The results showed that processing methods have an impact on the morphology of two subspecies of rice phytoliths, dry ashing producing more conjoined cell phytoliths or multicells phytoliths than acid extraction. Using a combination of both methods resulted in the presence of fewer conjoined cells than dry ashing alone, but more conjoined cells than acid extraction. Alternative explanations are proposed to explain the formation of conjoined phytolith cells. Microsc. Res. Tech. 75:1272–1276, 2012. © 2012 Wiley Periodicals, Inc.