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Bone marrow cells of swine: Collection and separation
Author(s) -
Branco Érika,
Cabral Rosa,
Gomes Bruno Duarte,
Kfoury José Roberto,
Miglino Maria Angelica
Publication year - 2012
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.22013
Subject(s) - bone marrow , iliac crest , haematopoiesis , mesenchymal stem cell , cell , peripheral blood mononuclear cell , medicine , pathology , biology , stem cell , cancer research , microbiology and biotechnology , in vitro , surgery , biochemistry
Bone marrow is a source of stem cells for greater and easier access, which is widely studied as a provider of hematopoietic and mesenchymal cells for various purposes, mainly therapeutic by the advances in research involving cell therapy. The swine is an animal species commonly used in the pursuit of development of experimental models. Thus, this study aimed to standardize protocol for collection and separation of bone marrow in swines, since this species is widely used as experimental models for various diseases. Twelve animals were used, which underwent bone marrow puncture with access from the iliac crest and cell separation by density gradient followed by a viability test with an average of 98% of viable cells. Given our results, we can ensure the swine as an excellent model for obtaining and isolation of mononuclear cells from bone marrow, stimulating several studies addressing the field of cell therapy. Microsc. Res. Tech., 2012. © 2012 Wiley Periodicals, Inc.