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Morphological characterization of the progenitor blood cells in canine and feline umbilical cord
Author(s) -
Brólio Marina P,
Vidane Atanásio S,
Zomer Helena D,
Wenceslau Cristiane V,
OzóRio Juliana J,
Martins Daniele S,
Miglino Maria A,
AmbróSio Carlos E
Publication year - 2012
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.21123
Subject(s) - umbilical cord , progenitor cell , progenitor , biology , placenta cord banking , pathology , andrology , anatomy , medicine , microbiology and biotechnology , stem cell , fetus , pregnancy , placenta , genetics
The umbilical cord blood (UCB) is an important source of hematopoietic stem cells with great deal of interest in regenerative medicine. The UCB cells have been extensively studied as an alternative to the bone marrow transplants. The challenge is to define specific methods to purify and characterize these cells in different animal species. This study is aimed at morphological characterization of progenitor cells derived from UCB highlighting relevant differences with peripheral blood of adult in dog and cats. Therefore, blood was collected from 18 dogs and 5 cats' umbilical cords from fetus in various developmental stages. The mononuclear cells were separated using the gradient of density Histopaque‐1077. Characterization of CD34+ cells was performed by flow cytometric analysis and transmission electron microscopy. Granulocytes (ancestry of the basophiles, eosinophiles, and neutrophiles) and agranulocytes (represented by immature lymphocytes) were identified. We showed for the first time the ultrastructural features of cat UCB cells. Microsc. Res. Tech. 75:766–770, 2012. © 2011 Wiley Periodicals, Inc.