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Quantitative 3D imaging of yeast by hard X‐ray tomography
Author(s) -
Zheng Ting,
Li Wenjie,
Guan Yong,
Song Xiangxia,
Xiong Ying,
Liu Gang,
Tian Yangchao
Publication year - 2012
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.21108
Subject(s) - tomography , yeast , computed tomography , biology , medicine , radiology , genetics
Full‐field hard X‐ray tomography could be used to obtain three‐dimensional (3D) nanoscale structures of biological samples. The image of the fission yeast, Schizosaccharomyces pombe , was clearly visualized based on Zernike phase contrast imaging technique and heavy metal staining method at a spatial resolution better than 50 nm at the energy of 8 keV. The distributions and shapes of the organelles during the cell cycle were clearly visualized and two types of organelle were distinguished. The results for cells during various phases were compared and the ratios of organelle volume to cell volume can be analyzed quantitatively. It showed that the ratios remained constant between growth and division phase and increased strongly in stationary phase, following the shape and size of two types of organelles changes. Our results demonstrated that hard X‐ray microscopy was a complementary method for imaging and revealing structural information for biological samples. Microsc. Res. Tech., 2012. © 2011 Wiley Periodicals, Inc.