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Morphological and morphometric characterization of agoutis' peripheral blood cells ( Dasyprocta prymnolopha , wagler, 1831) raised in captivity
Author(s) -
Júnior Airton Mendes Conde,
De Moura Fortes Eunice Anita,
De Menezes Danilo José Ayres,
De Oliveira Lopes Luana,
De Carvalho Maria Acelina Martins
Publication year - 2012
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.21066
Subject(s) - nucleus , basophilic , cytoplasm , anatomy , basophil , biology , pathology , pleomorphism (cytology) , platelet , microbiology and biotechnology , immunology , medicine , immunohistochemistry , immunoglobulin e , antibody
Thirty adult agoutis ( Dasyprocta primnolopha ) from the Nucleus of Study and Preservation of Wild Animals at the Federal University of Piauí were used. Blood scrubs of these animals were colored by the Leishman method and analyzed in light microscopy. The cells had been measured using programs that analyze images (Leica QWin – Image Processing and Analysis Software). Mature erythrocytes, basophil reticulocytes, lymphocytes, eosinophils, neutrophils, monocytes, and thrombocytes were identified. Agoutis' erythrocytes presented elliptical form, without nucleus with an average diameter of 5.64 micromeres ± 0.38. The lymphocytes are spherical cells with scarce cytoplasm, dense and with a very centralized rounded nucleus measuring an average diameter of 13.20 micromeres ± 0.35. The monocytes are slightly basophilic, with a spherical nucleus, central constriction, and an average diameter of 20.59 micromeres ± 0.32. The neutrophils are spherical, with a polymorphic lobulated nucleus, with an average diameter of 11.2 micromeres ± 0.20. The eosinophils are spherical with lobulated nucleus and with an average diameter of 14.25 micromeres ± 0.36. Only five basophils were observed, with abundance of cytoplasmic granules with 9.8 micrometers of diameter ± 0.30. Thrombocytopenic pleomorphism was frequent. There were similarities in the cellular constituents in peripheral blood of agoutis and of other rodents and humans. The cellular types from the peripheral blood, the morphology, and morphometry of the blood's cells did not vary according to sex. © Microsc. Res. Tech. 00:000?000, 2011. © 2011 Wiley Periodicals, Inc.