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Use of different morphological techniques to analyze the cellular composition of the adult zebrafish optic tectum
Author(s) -
Corbo Christopher P.,
Othman Nidaa A.,
Gutkin Michael C.,
Alonso Alejandra Del C.,
Fulop Zoltan L.
Publication year - 2012
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.21061
Subject(s) - danio , tectum , zebrafish , optic tectum , confocal , confocal microscopy , biology , osmium tetroxide , golgi apparatus , anatomy , electron microscope , chemistry , biophysics , microbiology and biotechnology , neuroscience , central nervous system , biochemistry , midbrain , optics , physics , endoplasmic reticulum , gene
Cellular composition of the adult zebrafish ( Danio rerio ) optic tectal cortex was examined in this study. Morphological techniques such as 1μm thick serial plastic sections stained with osmium tetroxide and toluidine blue, modified rapid Golgi silver impregnation, GFAP immunohistochemistry, confocal microscopy, as well as scanning and transmission electron microscopy were used. Neuronal and glial components are described and the layers of the cortex are revisited. Specific neuronal arrangements as well as unique glial/ependymal cells are described. A three dimensional rendering of the astrocytic fiber arrangement in the marginal zone is presented and a composite drawing summarizes the cellular composition of the optic tectum. Microsc. Res. Tech. 2011. © 2011 Wiley Periodicals, Inc.