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Entamoeba histolytica electrondense granules secretion in vitro and in vivo: Ultrastructural study
Author(s) -
ChÁvezmunguÍa Bibiana,
CastaÑÓN Guadalupe,
HernÁNdezRamÍRez VerÓNica,
GonzÁLezLÁZARO MÓNica,
TalamÁSRohana Patricia,
MartÍNezPalomo Adolfo
Publication year - 2012
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.21042
Subject(s) - entamoeba histolytica , hamster , in vivo , secretion , in vitro , collagenase , ultrastructure , biology , microbiology and biotechnology , chemistry , biochemistry , enzyme , anatomy
Electron dense granules (EDGs) were identified by transmission electron microscopy in Entamoeba histolytica trophozoites recovered from hamster liver lesions. Abundant granules were present in trophozoites recovered after 15 min of liver inoculation. Variation in the size and morphology of these EDGs was also observed. Numerous granules were present in the plasma membrane when these parasites were incubated for 5 min with MDCK monolayers. Release of these EDGs was suggested by the presence of granules in contact with the surface of the target cell plasma membrane. Parasite phagocytic invaginations were observed after 10 min of parasite‐monolayer interaction. In these structures, scarce granules were seen. Granules secretion was corroborated by obtaining of a pellet of these small structures from the incubation of trophozoites with collagen supernatant. Collagenase and gellatinase activity of this pellet was identified in SDS‐PAGE gels. EDGs were also present in amebic hamster liver lesions. Our observations corroborate that these granules are secreted and suggest that may participate in the cytopathic effect of E. histolytica both in vitro and in vivo. Microsc. Res. Tech., 2011. © 2011 Wiley Periodicals, Inc.