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Influence of low‐level laser associated with osteogenic proteins recombinant human BMP‐2 and Hevea brasiliensis on bone repair in Wistar rats
Author(s) -
Iyomasa Mamie Mizusaki,
Mardegan Issa João Paulo,
de Queiróz Tavares Mirella Lemos,
Lara Pereira Yamba Carla,
Sasso Stuani Maria Bernadete,
Mishima Fabíola,
CoutinhoNetto Joaquim,
Sebald Walter
Publication year - 2012
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.21033
Subject(s) - irradiation , laser , chemistry , recombinant dna , bone morphogenetic protein 2 , laser treatment , dentistry , medicine , in vitro , biochemistry , physics , nuclear physics , optics , gene
This study analyzed the newly formed bone tissue after application of recombinant human BMP‐2 (rhBMP‐2) and P‐1 (extracted from Hevea brasiliensis ) proteins, 2 weeks after the creation of a critical bone defect in male Wistar rats treated or not with a low‐intensity laser (GaAlAs 780 nm, 60 mW of power, and energy density dose of 30 J/cm 2 ). The animals were divided into two major groups: (1) bone defect plus low‐intensity laser treatment and (2) bone defect without laser irradiation. The following subgroups were also analyzed: (a) 5 μg of pure rhBMP‐2; (b) 5 μg of pure P‐1 fraction; (c) 5 μg of rhBMP‐2/monoolein gel; (d) 5 μg of P‐1 fraction/monoolein gel; (e) pure monoolein gel. Comparisons of the groups receiving laser treatment with those that did not receive laser irradiation show differences in the areas of new bone tissue. The group treated with 5 μg of rhBMP‐2 and laser irradiation was not significantly different ( P >0.05) than the nonirradiated group that received the same treatment. The irradiated, rhBMP‐2/monoolein gel treatment group showed a lower area of bone formation than the nonirradiated, rhBMP‐2/gel monoolein treatment group ( P < 0.001). The area of new bone tissue in the other nonirradiated and irradiated groups was not significantly different ( P > 0.05). Furthermore, the group that received the 5 μg of rhBMP‐2 application showed the greatest bone formation. We conclude that the laser treatment did not interfere with the area of new bone tissue growth and that the greatest stimulus for bone formation involved application of the rhBMP‐2 protein. Microsc. Res. Tech. 2011. © 2011 Wiley Periodicals, Inc.