Premium
Cytotoxicity of fipronil on mice liver cells
Author(s) -
De Oliveira Patrícia Rosa,
Bechara Gervásio Henrique,
Denardi Sandra Eloisi,
Oliveira Rodrigo Juliano,
Mathias Maria Izabel Camargo
Publication year - 2012
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.21018
Subject(s) - fipronil , hepatocyte , vacuole , necrosis , cytotoxic t cell , cytotoxicity , andrology , biology , steatosis , cytoplasm , chemistry , pathology , endocrinology , biochemistry , in vitro , pesticide , medicine , agronomy
In the present study, mice livers were examined following exposure to different doses of fipronil (15, 25, and 50 mg/kg). Histological and histochemical techniques were used to determine the cytotoxic potential of this compound and to assess the damage it caused to livers. Mice were divided into four groups: control group and groups I, II, and III were exposed to 15, 25, and 50 mg/kg fipronil, respectively. Our findings revealed cytological, morphohistological, and histochemical alterations in liver cells of animals from groups I, II, and III compared to group control animals. These changes included Kupffer‐cell proliferation, hepatocyte hypertrophy, accumulation and distribution of proteins, polysaccharides, lipids, and vacuoles in the cytoplasm of hepatocytes, and congestion of blood vessels. These phenotypes mainly characterize the following: (a) autophagic processes, (b) steatosis, and (c) cell death by necrosis, which demonstrate the damage caused by fipronil on nontarget organisms in artificial conditions. Microsc. Res. Tech., 2012. © 2011 Wiley Periodicals, Inc.