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Single molecule microscopy methods for the study of DNA origami structures
Author(s) -
Birkedal Victoria,
Dong Mingdong,
Golas Monika M.,
Sander Bjoern,
Andersen Ebbe Sloth,
Gothelf Kurt Vesterager,
Besenbacher Flemming,
Kjems Jørgen
Publication year - 2011
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.20962
Subject(s) - dna origami , förster resonance energy transfer , microscopy , nanotechnology , single molecule experiment , fluorescence microscope , nanostructure , transmission electron microscopy , force spectroscopy , molecule , materials science , dna nanotechnology , atomic force microscopy , chemistry , dna , fluorescence , physics , optics , biochemistry , organic chemistry
Single molecule microscopy techniques play an important role in the investigation of advanced DNA structures such as those created by the DNA origami method. Three single molecule microscopy techniques are particularly interesting for the investigation of complex self‐assembled three‐dimensional (3D) DNA nanostructures, namely single molecule fluorescence microscopy, atomic force microscopy (AFM), and cryogenic transmission electron microscopy (cryo‐EM). Here we discuss the strengths of these three techniques and demonstrate how their interplay can yield very important and unique new insights into the structure and conformation of advanced biological nanostructures. The applications of the three single molecule microscopy techniques are illustrated by focusing on a self‐assembled DNA origami 3D box nanostructure. Its size and structure were studied by AFM and cryo‐EM, while the lid opening, which can be controlled by the addition of oligonucleotide keys, was recorded by Förster/fluorescence resonance energy transfer (FRET) spectroscopy. Microsc. Res. Tech., 2011. © 2010 Wiley‐Liss, Inc.