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Influence of Er:YAG laser frequency on dentin caries removal capacity
Author(s) -
RaucciNeto Walter,
Chinelatti Michelle Alexandra,
Ito Izabel Yoko,
Pécora Jesus Djalma,
PalmaDibb Regina Guenka
Publication year - 2011
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.20902
Subject(s) - dentin , molar , scanning electron microscope , laser , materials science , irradiation , dentistry , biomedical engineering , chemistry , composite material , optics , medicine , physics , nuclear physics
The purposes of this study were to evaluate in vitro the influence of different frequencies of Er:YAG laser on the human dentin caries removal capacity. Thirty fragments obtained from third molars were randomly assigned into three groups ( n = 10) according to the laser frequency used: 4, 6, and 10 Hz. The caries lesion (±1 mm deep) was induced before the irradiation by S.mutans cultures for 6 weeks. The specimens of all groups were irradiated with 200 mJ of energy in noncontact and focused mode under constant refrigeration (water flow: 2.5 mL/min). Quantitative analysis of the caries removal was performed by DIAGNOdent TM and the Axion Vision TM software. Qualitative analysis was performed by Scanning electron microscope (SEM) and light microscope (LM). Data were analyzed by ANOVA and Fishers' tests. The DIAGNOdent TM revealed that the caries removal was similar with 4 and 6 Hz and was superior with 10 Hz ( P < 0.05). The analysis with Axion Vision TM software revealed that the caries removal was similar with 6 and 10 Hz and the 4 Hz group promoted the lowest caries removal. Through SEM morphologic analysis, some specimens irradiated with 4 Hz presented, under the demineralized dentin, a disorganized collagenous matrix. The LM images revealed that all frequencies used promoted irregular caries removal, being observed over preparations with 6 and 10 Hz. It can be concluded that the increase of Er:YAG laser frequency provided a higher dentin caries removal without selectivity to the disorganized dentin. Microsc. Res. Tech., 2010. © 2010 Wiley‐Liss, Inc.