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Disclosure in 3D of slit‐membrane as well as ‐strands, and en‐face basal lamina in situ of renal glomerulus of normal rats in embedment‐free section transmission electron microscopy
Author(s) -
Kondo Hisatake
Publication year - 2011
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.20883
Subject(s) - basal lamina , transmission electron microscopy , electron microscope , slit , anatomy , ultrastructure , glomerulus , microscopy , in situ , biophysics , embedment , chemistry , materials science , optics , kidney , biology , nanotechnology , composite material , physics , organic chemistry , endocrinology
With higher contrast and transparency due to the absence of epon and stereo‐viewing effect due to thicker sections than conventional electron microscopy as methodological advantages, the renal glomerular slits were re‐examined in embedment‐free section electron microscopy. In addition to clear demonstration of strands bridging the slits in forms of ladders with highly irregular intervals and various extension‐directions and length, this study disclosed clearly for the first time in the “section” TEM thin sheets which partially spanned the slit together with the strand‐ladders. No strands were found to align in forms of typical zippers in normal kidney. Furthermore, en‐face ultrastructure of the basal lamina in situ was clearly demonstrated in superimposed sites of the endothelial fenestrae with the slits. Microsc. Res. Tech. 74:142–147, 2011. © 2010 Wiley‐Liss, Inc.