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A comparison of imaging methodologies for 3D tissue engineering
Author(s) -
Smith Louise E.,
Smallwood Rod,
Macneil Sheila
Publication year - 2010
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.20859
Subject(s) - optical coherence tomography , microscopy , biomedical engineering , confocal microscopy , confocal , confocal laser scanning microscopy , phase contrast microscopy , fluorescence microscope , medical imaging , materials science , computer science , pathology , biology , optics , medicine , artificial intelligence , fluorescence , microbiology and biotechnology , physics
Imaging of cells in two dimensions is routinely performed within cell biology and tissue engineering laboratories. When biology moves into three dimensions imaging becomes more challenging, especially when multiple cell types are used. This review compares imaging techniques used regularly in our laboratory in the culture of cells in both two and three dimensions. The techniques reviewed include phase contrast microscopy, fluorescent microscopy, confocal laser scanning microscopy, electron microscopy, and optical coherence tomography. We compare these techniques to the current “gold standard” for imaging three‐dimensional tissue engineered constructs, histology. Microsc. Res. Tech. 73:1123–1133, 2010. © 2010 Wiley‐Liss, Inc.