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3D reconstruction of histological sections: Application to mammary gland tissue
Author(s) -
ArgandaCarreras Ignacio,
FernándezGonzález Rodrigo,
MuñozBarrutia Arrate,
OrtizDeSolorzano Carlos
Publication year - 2010
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.20829
Subject(s) - computer science , high resolution , 3d reconstruction , mammary gland , annotation , artificial intelligence , resolution (logic) , computer vision , biomedical engineering , biology , cancer , geology , medicine , remote sensing , breast cancer , genetics
In this article, we present a novel method for the automatic 3D reconstruction of thick tissue blocks from 2D histological sections. The algorithm completes a high‐content (multiscale, multifeature) imaging system for simultaneous morphological and molecular analysis of thick tissue samples. This computer‐based system integrates image acquisition, annotation, registration, and three‐dimensional reconstruction. We present an experimental validation of this tool using both synthetic and real data. In particular, we present the 3D reconstruction of an entire mouse mammary gland and demonstrate the integration of high‐resolution molecular data. Microsc. Res. Tech. 73:1019–1029, 2010. © 2010 Wiley‐Liss, Inc.