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Histological analysis of the alterations on cortical bone channels network after radiotherapy: A rabbit study
Author(s) -
Rabelo Gustavo Davi,
Beletti Marcelo Emílio,
Dechichi Paula
Publication year - 2010
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.20826
Subject(s) - lacunarity , tibia , cortical bone , bone matrix , region of interest , matrix (chemical analysis) , rabbit (cipher) , anatomy , medicine , nuclear medicine , chemistry , mathematics , radiology , mathematical analysis , cartilage , statistics , fractal dimension , chromatography , fractal
The aim of this study was to evaluate the effects of radiotherapy in cortical bone channels network. Fourteen rabbits were divided in two groups and test group received single dose of 15 Gy cobalt‐60 radiation in tibia, bilaterally. The animals were sacrificed and a segment of tibia was removed and histologically processed. Histological images were taken and had their bone channels segmented and called regions of interest (ROI). Images were analyzed through developed algorithms using the SCILAB mathematical environment, getting percentage of bone matrix, ROI areas, ROI perimeters, their standard deviations and Lacunarity. The osteocytes and empty lacunae were also counted. Data were evaluated using Kolmogorov‐Smirnov, Mann Whitney, and Student's t test ( P < 0.05). Significant differences in bone matrix percentage, area and perimeters of the channels, their respective standard deviations and lacunarity were found between groups. In conclusion, the radiotherapy causes reduction of bone matrix and modifies the morphology of bone channels network. Microsc. Res. Tech. 73:1015–1018, 2010. © 2010 Wiley‐Liss, Inc.