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Oogenesis and programmed cell death of nurse cells in the endoparasitoid, Pteromalus puparum
Author(s) -
Dong ShengZhang,
Ye GongYin,
Guo JianYang,
Yu XiaoPing,
Hu Cui
Publication year - 2010
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.20808
Subject(s) - oogenesis , nurse cell , oocyte , microbiology and biotechnology , biology , programmed cell death , yolk , apoptosis , biochemistry , embryo , ecology
In this study, we describe the features of oogenesis in the endoparasitoid, Pteromalus puparum , as well as the distinct type of programmed cell death of the nurse cells through conventional light and fluorescent markers for apoptosis and immunofluorescent analysis. Oogenesis in this endoparasitoid is divided into five stages, of which stages 1–2, 3–4, and 5 are corresponding to previtellogenic growth, yolk uptake, and the formation of egg envelopes, respectively. From these studies, we demonstrate two critical events, which are vitellogenin absorption and rapid transfer of nurse cell content, resulting in remarkable increase in the volume of oocytes during oogenesis in this endoparasitoid. Vitellogenin absorption initiates in the oocyte of early stage 3, and bulk transfer of nurse cell content into the oocyte occurs at stage 4 of oogenesis in P . puparum , which is mainly characterized with the programmed cell death in the nurse cell complex. Microsc. Res. Tech., 2010. © 2009 Wiley‐Liss, Inc.