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Expression profile of a 400‐bp Stra8 promoter region during spermatogenesis
Author(s) -
Antonangeli Fabrizio,
Giampietri Claudia,
Petrungaro Simonetta,
Filippini Antonio,
Ziparo Elio
Publication year - 2009
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.20724
Subject(s) - biology , transgene , green fluorescent protein , microbiology and biotechnology , gene , spermatogenesis , germ cell , retinoic acid , genetically modified mouse , genetics , endocrinology
Although numerous markers have been helpful in isolating and enriching spermatogonial stem cells (SSCs), such as Thy‐1 and GFRα‐1, no specific marker for this cell type has been identified so far. A 400‐bp regulatory region of the stimulated by retinoic acid gene 8 ( Stra8 ) promoter was reported to direct gene expression into SSCs and we have recently generated a new transgenic mouse model harboring the enhanced green fluorescent protein (EGFP) downstream of this Stra8 promoter. In this study, a detailed analysis of the EGFP expression pattern in the testis was carried out, showing that the transgene was expressed in meiotic and postmeiotic germ cells and not in undifferentiated germ cells. These findings were supported by confocal microscopy and flow cytometric analyses, and do not agree with the previous report concerning the 400‐bp Stra8 promoter activity. Microsc. Res. Tech. 2009. © 2009 Wiley‐Liss, Inc.