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Ultrastructural observations on tegumental surface of Raillietina echinobothrida and its alterations caused by root‐peel extract of Millettia pachycarpa
Author(s) -
Roy Bishnupada,
Dasgupta Shyamashree,
Tandon Veena
Publication year - 2008
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.20623
Subject(s) - biology , vacuolization , anthelmintic , hymenolepis diminuta , parasite hosting , traditional medicine , botany , basal lamina , ultrastructure , helminths , cestoda , immunology , zoology , medicine , world wide web , computer science , endocrinology
Millettia pachycarpa Benth (Leguminosae) has a usage in traditional medicine system practiced among the Lushai tribes of Mizoram, a state in North East India, who customarily consume the aqueous extract of the root peel of the plant to get rid of intestinal worm infections. The crude ethanol, methanol, and acetone fractions of the plant were assayed against Raillietina echinobothrida , the intestinal cestode parasite of domestic fowl, to authenticate the putative anthelmintic efficacy and cestocidal potential in particular of the plants. In vitro exposure of the worm to the extract at a concentration of 25 mg/mL phosphate buffered saline (at 37°C ± 1°C) revealed distortion and disruption of mitochondria, nucleus, nucleolus, nuclear membrane, basal lamina, and tegumental vacuolization in the distal cytoplasm leading to scar formation in the surface. The possible use of the plant as a potential anthelmintic against cestode parasite is discussed. Microsc. Res. Tech., 2008. © 2008 Wiley‐Liss, Inc.