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Saponaria officinalis karyology and karyotype by means of image analyzer and atomic force microscopy
Author(s) -
Di Bucchianico S.,
Venora G.,
Lucretti S.,
Limongi T.,
Palladino L.,
Poma A.
Publication year - 2008
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.20613
Subject(s) - karyotype , biology , population , nuclear dna , chromosome , officinalis , ploidy , dna , chromosome number , cytotaxonomy , fluorescence microscope , microbiology and biotechnology , genetics , fluorescence , botany , mitochondrial dna , physics , gene , demography , sociology , quantum mechanics
Abstract The aim of this work was to offer a contribution to the characterization of taxonomic entity of Saponaria officinalis (2 n = 28; an herbaceous perennial species; saporin, a type 1 Ribosome Inactivating Protein, is present in leaves and seeds) by a cytogenetic and karyomorphological approach. We investigated the karyotype's morphometry correlated with Stebbin's symmetric index; the same information has been used for computing the indices resemblance between chromosomes (REC), symmetric indices (SYI), and total form (TF%) which allow the comparison between species and evaluation of karyological evolution. Fluorescence intensities of the stained nuclei were measured by a flow cytometer and, for the first time, values for nuclear DNA content were estimated by comparing nuclei fluorescence intensities of the test population with those of appropriate internal DNA standards. Our study is also aimed to introduce chromosomal volumes, which were determined by atomic force microscopy (AFM), as novel karyomorphological parameter which could allow for chromosome discrimination especially when tiny ones are present. Microsc. Res. Tech., 2008. © 2008 Wiley‐Liss, Inc.