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Immunohistochemical characterization of adenosine receptors in rat aorta and tail arteries
Author(s) -
Leal Sandra,
Sá Carlos,
Gonçalves Jorge,
Fresco Paula,
Diniz Carmen
Publication year - 2008
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.20609
Subject(s) - adventitia , adenosine receptor , receptor , aorta , adenosine , immunohistochemistry , abdominal aorta , pathology , biology , artery , anatomy , chemistry , medicine , endocrinology , agonist
Adenosine plays an important role in the cardiovascular system, activating adenosine A 1 , A 2A , A 2B , and A 3 receptors, and regulating blood flow either by acting directly on vascular cells or indirectly because of its effects on the central or peripheral nervous systems. The aim of the present study was to investigate whether the pattern of distribution of adenosine receptor subtypes is different on elastic and muscular, using abdominal aorta and tail arteries as models. Immunohistochemistry using anti‐A 1 , anti‐A 2A , anti‐A 2B , and anti‐A 3 receptor antibodies was performed on perfused‐fixed/paraffin‐embedded arteries from Wistar rats. 3,3′‐Diaminobenzidine tetrahydrochloride (DAB; activated by hydrogen peroxide) staining revealed significant differences in the abundance of A 1 , A 2A , and A 3 receptors between abdominal aorta and tail artery and allowed the identification of distinct distribution patterns for A 1 , A 2A , A 2B , and A 3 receptors in the tunica adventitia, media, and intima of muscular and elastic arteries. Data are compatible with several previous functional reports supporting that different adenosine receptor subtype expression and/or their distribution in the vessel wall may influence their respective contribution to the control of blood flow. Microsc. Res. Tech., 2008. © 2008 Wiley‐Liss, Inc.