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Low‐voltage transmission electron microscopy reveals SV40 viral particles within secretory granules in pancreatic cells
Author(s) -
Bendayan Moise,
Gingras Diane,
Ziv Ehud,
Haviv Yosef S.
Publication year - 2008
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.20603
Subject(s) - transmission electron microscopy , immunocytochemistry , electron microscope , biophysics , microbiology and biotechnology , microscopy , chemistry , biology , materials science , nanotechnology , optics , physics , endocrinology
Novel approach in low voltage transmission electron microscopy (TEM) has revealed the presence of SV40 viral like particles in the secretory zymogen granules and in spherical membrane‐bound dense bodies of SV40 infected pancreatic cells. The presence of SV40 antigen in these cellular compartments was confirmed by immunocytochemistry of the VP1 antigen. Visualization of the viral particles was only possible by examining ultrathin tissue sections with low‐voltage TEM that significantly enhances imaging contrast. Results indicate that following infection of the cell entry and trafficking of the viral particles are present in unique cellular compartments such as ER, dense bodies, and secretory granules. Microsc. Res. Tech., 2008. © 2008 Wiley‐Liss, Inc.