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X‐ray imaging of various biological samples using a phase‐contrast hard X‐ray microscope
Author(s) -
Kim Guk Bae,
Yoon Yae Jin,
Shin Tae Joo,
Youn Hwa Shik,
Gho Yong Song,
Lee Sang Joon
Publication year - 2008
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.20601
Subject(s) - microscope , electron microscope , resolution (logic) , microscopy , optical microscope , optics , materials science , 4pi microscope , scanning electron microscope , biological specimen , x ray , phase contrast microscopy , conventional transmission electron microscope , scanning transmission electron microscopy , physics , computer science , artificial intelligence
Abstract In this study, we visualized the internal structures of various bio‐samples and found the optimum conditions of test samples for the 7 keV hard X‐ray microscope of the Pohang light source. From the captured X‐ray images, we could observe the intercellular and intracellular structures of dehydrated human cells and mouse tumor tissues without using any staining materials in a spatial resolution better than 100 nm. The metastasized lung tissue, which was several tens of micrometers in thickness, was found to be very well suited to this hard X‐ray microscope system, because it is nearly impossible to observe such a nontransparent and thick sample with a high spatial resolution better than 100 nm using any microscopes such as a soft X‐ray microscope, an optical microscope, or an electron microscope. Microsc. Res. Tech., 2008. © 2008 Wiley‐Liss, Inc.