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Ultrastructure of Trypanosoma cruzi revisited by atomic force microscopy
Author(s) -
Rocha Gustavo Miranda,
Miranda Kildare,
Weissmüller Gilberto,
Bisch Paulo Mascarello,
de Souza Wanderley
Publication year - 2008
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.20535
Subject(s) - flagellum , axoneme , ultrastructure , trypanosoma cruzi , transmission electron microscopy , basal body , biology , biophysics , microscopy , electron microscope , atomic force microscopy , cytoskeleton , microbiology and biotechnology , nanotechnology , anatomy , materials science , optics , bacteria , physics , cell , parasite hosting , biochemistry , genetics , world wide web , computer science
Abstract Most advances in atomic force microscopy (AFM) have been accomplished in recent years. Previous attempts to use AFM to analyze the organization of pathogenic protozoa did not significantly contribute with new structural information. In this work, we introduce a new perspective to the study of the ultrastructure of the epimastigote form of Trypanosoma cruzi by AFM. Images were compared with those obtained using field emission scanning electron microscopy of critical point dried cells and transmission electron microscopy of negative stained detergent‐extracted and air‐dried cells. AFM images of epimastigote forms showed a flagellum furrow separating the axoneme from the paraflagellar rod (PFR) present from the emergence of the flagellar pocket to the tip of the flagellum. At high magnification, a row of periodically organized structures, which probably correspond to the link between the axoneme, the PFR and the flagellar membrane were seen along the furrow. In the origin of the flagellum, two basal bodies were identified. Beyond the basal bodies, small periodically arranged protrusions, positioned at 400 nm from the flagellar basis were seen. This structure was formed by nine substructures distributed around the flagellar circumference and may correspond to the flagellar necklace. Altogether, our results demonstrate the importance of the application of AFM in the structural characterization of the surface components and cytoskeleton on protozoan parasites. Microsc. Res. Tech., 2008. © 2007 Wiley‐Liss, Inc.