Premium
Bioimaging TOF‐SIMS: High resolution 3D imaging of single cells
Author(s) -
Nygren Håkan,
Hagenhoff Birgit,
Malmberg Per,
Nilsson Mikael,
Richter Katrin
Publication year - 2007
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.20502
Subject(s) - chemistry , phosphocholine , membrane , ion , potassium , phospholipid , secondary ion mass spectrometry , mass spectrometry imaging , fragmentation (computing) , biophysics , sodium , analytical chemistry (journal) , mass spectrometry , biochemistry , chromatography , biology , ecology , organic chemistry , phosphatidylcholine
The distribution of phosphocholine ions ( m/z 184, m/z 86), sodium ions, and potassium ions in thyroid tumor cells was analyzed by imaging TOF‐SIMS. Repeated sputtering with a C 60 +source and subsequent analysis with a Bi 3 +gun produced a series of 138 images that were stacked to make a 3D display of the chemistry of cells. Phosphocholine was seen in the plasma membrane ( m / z 184) and intracellular membranes ( m/z 86). The different fragmentation of the phospholipid probably reflects the chemical composition of membranes at these sites. High intensity of secondary ion signals of potassium was seen in membrane‐encompassed cellular compartments. The data indicate that potassium ions are compartmentalized in thyroid tumor cells. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.