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Clinical two‐photon microendoscopy
Author(s) -
König K.,
Ehlers A.,
Riemann I.,
Schenkl S.,
Bückle R.,
Kaatz M.
Publication year - 2007
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.20445
Subject(s) - elastin , autofluorescence , two photon excitation microscopy , endoscope , in vivo , dermis , biomedical engineering , medicine , lens (geology) , extracellular matrix , preclinical imaging , multiphoton fluorescence microscope , pathology , radiology , optics , chemistry , fluorescence , fluorescence microscope , biology , biochemistry , physics , microbiology and biotechnology
Two‐photon medical imaging has found its way into dermatology as an excellent method for noninvasive skin cancer detection without need of contrast agents as well as for in situ drug screening of topically‐applied cosmetical and pharmaceutical components. There is an increasing demand to apply the multiphoton technology also for deep‐tissue skin imaging as well as for intracorporal imaging. We report on the first clinical use of multiphoton endoscopes, in particular of a miniaturized rigid two‐photon GRIN lens endoscope. The microendoscope was attached to the multiphoton tomograph DermaInspect and employed to detect the extracellular matrix proteins collagen and elastin in the human dermis of volunteers and patients with ulcera by in vivo second harmonic generation and in vivo two‐photon autofluorescence. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.