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Fluorescence detection with high time resolution: From optical microscopy to simultaneous force and fluorescence spectroscopy
Author(s) -
Gaiduk Alexander,
Kühnemuth Ralf,
Felekyan Suren,
Antonik Matthew,
Becker Wolfgang,
Kudryavtsev Volodymyr,
Sandhagen Carl,
Seidel Claus A.M.
Publication year - 2007
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.20430
Subject(s) - picosecond , fluorescence , microscopy , fluorescence cross correlation spectroscopy , fluorescence microscope , resolution (logic) , microscope , fluorescence correlation spectroscopy , laser induced fluorescence , fluorescence lifetime imaging microscopy , photon counting , fluorescence spectroscopy , signal (programming language) , temporal resolution , optics , materials science , two photon excitation microscopy , laser , photon , physics , computer science , artificial intelligence , programming language
Picosecond time‐resolution fluorescence signal detection over many hours is possible using the time‐correlated single photon counting (TCSPC) technique. Advanced TCSPC with clock oscillator set by the pulsed laser and data analysis provides a tool to investigate processes in single molecules on time scale from picoseconds to seconds. Optical imaging techniques combined with TCSPC allow one to study the spatial distribution of fluorescence properties in solution and on a surface. Mechanical manipulation of a single macromolecule by means of an atomic‐force microscope makes it possible to detect fluorescence signal changes as a function of mechanical conformations of a fluorescent dye attached to a single DNA molecule. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.