Premium
Multiphoton fluorescence lifetime imaging of human hair
Author(s) -
Ehlers Alexander,
Riemann Iris,
Stark Martin,
König Karsten
Publication year - 2007
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.20395
Subject(s) - fluorescence , fluorescence lifetime imaging microscopy , autofluorescence , two photon excitation microscopy , femtosecond , excitation , chemistry , optics , laser , materials science , biophysics , physics , biology , quantum mechanics
In vivo and in vitro multiphoton imaging was used to perform high resolution optical sectioning of human hair by nonlinear excitation of endogenous as well as exogenous fluorophores. Multiphoton fluorescence lifetime imaging (FLIM) based on time‐resolved single photon counting and near‐infrared femtosecond laser pulse excitation was employed to analyze the various fluorescent hair components. Time‐resolved multiphoton imaging of intratissue pigments has the potential (i) to identify endogenous keratin and melanin, (ii) to obtain information on intrahair dye accumulation, (iii) to study bleaching effects, and (iv) to monitor the intratissue diffusion of pharmaceutical and cosmetical components along hair shafts. Microsc. Res. Tech. 2007. © 2006 Wiley‐Liss, Inc.