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Intranuclear paracrystals observed in striated muscle specific LIM protein‐deficient mouse cardiomyocytes
Author(s) -
Muto M.,
Sargeant A.,
Tangkawattana P.,
Takeyasu K.,
Nishijima Y.,
Bonagura J.,
Hoshijima M.,
Nakade T.,
Hosaka Y.,
Faulkner G.,
Tomono K.,
Yamaguchi M.
Publication year - 2007
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.20386
Subject(s) - paracrystalline , myofibril , rna , biology , ultrastructure , biophysics , microbiology and biotechnology , anatomy , chemistry , crystallography , biochemistry , gene
A paracrystalline structure was observed within left ventricular cardiomyocyte nuclei of MLP −/− mice. The paracrystal possessed cross lines, ∼8.0 μm long and 0.3 μm wide, with a slender spindle shape and a periodicity of 13 nm. Paracrystals were best observed along the longitudinal orientation of myofibrils and were detected in less than 10% of the nuclei observed. One dimension of the protein unit forming the paracrystal was 8.5 nm long. The electron density of the paracrystal appeared to be slightly higher than that of heterochromatin, suggesting that RNA‐associated proteins are constituents of the paracrystal. This is the first report of intranuclear paracrystals in cardiomyocytes, which appear to be unique to MLP −/− mice. Microsc. Res. Tech. 2007. © 2006 Wiley‐Liss, Inc.