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Electron microscopic study of novel threadlike structures on the surfaces of mammalian organs
Author(s) -
Lee ByungCheon,
Yoo Jung Sun,
Ogay Vyacheslav,
Kim Ki Woo,
Dobberstein Harald,
Soh KwangSup,
Chang ByungSoo
Publication year - 2007
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.20383
Subject(s) - transmission electron microscopy , scanning electron microscope , electron microscope , materials science , anatomy , focused ion beam , extracellular matrix , ultrastructure , chemistry , optics , biology , composite material , nanotechnology , microbiology and biotechnology , physics , ion , organic chemistry
The ultrastructures of novel threadlike structures (NTSs) and corpuscles on the surfaces of internal organs of rats were investigated using electron microscopy. The samples were studied in situ by using a stereomicroscope and were taken for further morphological analysis. Scanning electron microscope (SEM) images revealed a bundle structure of threadlike tissue, which was composed of several 10‐μm‐thick subducts. The surfaces of the corpuscles were rather coarse and fenestrated. The corpuscles had cucumber‐like shapes with an average length of about 2 mm and a thickness of about 400 μm. Transmission electron microscope (TEM) images disclosed disordered collagen fibers, which formed the extracellular matrix of the threadlike tissue, and immune‐function cells, like macrophages, mast cells, and eosinophils. Sinuses of various diameters, which were thought to be cross‐sections of the lumens of the subducts, were observed in the TEM, cryo‐SEM and focused‐ion‐beam SEM images. These SEM images were obtained for the first time to reveal the detailed structure of the NTSs that were only recently discovered. Microsc. Res. Tech., 2006. © 2006 Wiley‐Liss, Inc.