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Fluorescent proteins as markers in the plant secretory pathway
Author(s) -
Hanton Sally L.,
Brandizzi Federica
Publication year - 2006
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.20276
Subject(s) - organelle , biology , secretory protein , microbiology and biotechnology , fluorescence , computational biology , cell , secretion , biochemistry , physics , quantum mechanics
The use of fluorescent proteins and live cell imaging has greatly increased our knowledge of cell biology in recent years. Not only can these technologies be used to study protein trafficking under different conditions, but they have also been of use in elucidating the relationships between different organelles in a noninvasive manner. The use of multiple different fluorochromes allows the observation of interactions between organelles and between proteins, making this one of the fastest‐developing and exciting fields at this time. In this review, we discuss the multitude of fluorescent markers that have been generated to study the plant secretory pathway. Although these markers have been used to solve many mysteries in this field, some areas that require further discussion remain. Microsc. Res. Tech. 69:152–159, 2006. © 2006 Wiley‐Liss, Inc.