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Identification of mineralized elastic fibers on wet samples by SEM
Author(s) -
Boraldi Federica,
Tonelli Massimo,
Gheduzzi Dealba,
Ronchetti Ivonne Pasquali,
Quaglino Daniela
Publication year - 2005
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.20212
Subject(s) - pseudoxanthoma elasticum , microanalysis , ultrastructure , connective tissue , electron probe microanalysis , pathology , materials science , mineralogy , identification (biology) , chemistry , analytical chemistry (journal) , scanning electron microscope , composite material , chromatography , biology , medicine , botany , organic chemistry
A method is described that could be of potential use for the rapid ultrastructural identification of abnormal and fragmented elastic fibers in very small wet samples of dermal biopsies from patients affected by Pseudoxanthoma elasticum (PXE). Moreover, the method, which consists of the use of sealed capsules transparent to electrons, allows the rapid and accurate localization and detection of mineralized areas in PXE patients and of their ion composition by X‐ray microanalysis. This methodology could be of great help in any tissue disorder, especially in connective tissue disorders, characterized by structural alterations associated with ion precipitation. Microsc. Res. Tech. 67:296–299, 2005. © 2005 Wiley‐Liss, Inc.