Methods for characterizing plant fibers | Zendy

Cruthers Natasha | Zendy; Carr Debra | Zendy; Niven Brian | Zendy; Girvan Elizabeth | Zendy; Laing Raechel | Zendy

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Methods for characterizing plant fibers

Author(s) -

Cruthers Natasha,

Carr Debra,

Niven Brian,

Girvan Elizabeth,

Laing Raechel

Publication year - 2005

Publication title -

microscopy research and technique

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.536

H-Index - 118

eISSN - 1097-0029

pISSN - 1059-910X

DOI - 10.1002/jemt.20206

Subject(s) - tenax , cultivar , microscopy , scanning electron microscope , materials science , optical microscope , composite material , horticulture , chemistry , biology , optics , chromatography , physics , gas chromatography

The effectiveness of different microscopy techniques for measuring the dimensions of ultimate fibers from harakeke ( Phormium tenax , New Zealand flax) was investigated using a factorial experimental design. Constant variables were geographical location, location of specimens along the leaf, season (winter), individual plant, a fourth leaf from a north‐facing fan, age of plant, and cultivars (two). Experimental variables were microscopy techniques and measurement axis. Measurements of width and length of harakeke ultimate fibers depended on the microscopic preparation/technique used as well as the cultivar examined. The best methods were (i) transverse sections of leaf specimens 4 μm thick, embedded in Paraplast® and observed using light microscopy, and (ii) nonfixed ultimate fibers observed using scanning electron microscopy. Microsc. Res. Tech. 67:260–264, 2005. © 2005 Wiley‐Liss, Inc.

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