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Ultrastructure of in situ perfusion‐fixed avian liver, with special reference to structure of the sinusoids
Author(s) -
Ghoddusi Majid,
Kelly W. Roger
Publication year - 2004
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.20107
Subject(s) - ultrastructure , in situ , pathology , perfusion , biology , anatomy , chemistry , medicine , organic chemistry
Broiler chicken and laying hen livers were fixed using a simple technique of in situ puncture perfusion of cacodylate‐buffered fixative, which allowed characterisation of the fine structure of hepatic parenchyma, hepatocytes, bile ductules, and, in particular, the sinusoidal cells including endothelial, Kupffer, and Ito cells. Sinusoidal endothelial cells with their bulging perinuclear cytoplasm, evident in both transmission and scanning electron micrographs, were easily distinguishable from Kupffer cells, which possessed numerous pseudopodia. Bile ductular epithelium and hepatocytes of the laying hens contained large amounts of lipid. The ultrastructural characteristics of intercalated cells (putative extra‐sinusoidal macrophages of chicken liver) are described and their possible role as precursors of Kupffer cells is discussed. Microsc. Res. Tech. 65:101–111, 2004. © 2004 Wiley‐Liss, Inc.