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Alignment of the cell nucleus from labeled proteins only for 4D in vivo imaging
Author(s) -
Rieger B.,
Molenaar C.,
Dirks R.W.,
Van Vliet L.J.
Publication year - 2004
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.20069
Subject(s) - translation (biology) , coordinate system , rotation (mathematics) , biological system , nucleus , nuclear matrix , confocal microscopy , confocal , a priori and a posteriori , biophysics , chemistry , computer science , physics , artificial intelligence , biology , microbiology and biotechnology , optics , dna , messenger rna , biochemistry , chromatin , gene , philosophy , epistemology
Studies of protein dynamics by 4D (3D + time) confocal microscopy in vivo are hampered by global cell motion. The time between the acquisitions of the 3D images is in the order of minutes. Therefore, it is not to be expected that the cell as a whole remains fixed in the water basin on the stage. This superimposes a motion on the protein dynamics that has to be removed. We present a robust registration technique to align the cell images that does not require the a priori establishment of point‐to‐point correspondences. Instead, it uses the distribution of the labeled proteins. After correction for the translation, the 3D rotation of the cell is estimated. A robust intrinsic body coordinate system is constructed via the inertia tensor from the intensity distribution. By combining basis transformation to this intrinsic coordinate system, we can calculated the rotation matrix in a conceptual and computational straightforward manner. We have evaluated the performance of this approach in three experiments with human osteaosarcoma cells (U‐2 OS), where the nuclear proteins Histon H4 and PML were visualized. The PML is concentrated in several dozen nuclear spots. Expression of Histon H4 results in a total nuclear staining. The registration results for both channels computed independently are very similar. Practically, this means that only the labeled material needs to be observed and still registration of the cell as a whole can be achieved. Microsc. Res. Tech. 64:142–150, 2004. © 2004 Wiley‐Liss, Inc.