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Expression mosaic of odorant‐binding proteins in Drosophila olfactory organs
Author(s) -
Shanbhag S.R.,
HekmatScafe D.,
Kim M.S.,
Park S.K.,
Carlson J.R.,
Pikielny C.,
Smith D.P.,
Steinbrecht R.A.
Publication year - 2001
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.1179
Subject(s) - sensillum , odorant binding protein , biology , drosophila melanogaster , insect , antenna (radio) , drosophila (subgenus) , gene , genome , microbiology and biotechnology , anatomy , genetics , botany , telecommunications , computer science
Deciphering the genome of the fruitfly, Drosophila melanogaster, has revealed 39 genes coding for putative odorant‐binding proteins (OBPs), more than are known at present for any other insect species. Using specific antibodies, the expression mosaic of five such OBPs (OS‐E, OS‐F, LUSH, PBPRP2, PBPRP5) on the antenna and maxillary palp has been mapped in the electron microscope. It was found that (1) OBP expression does correlate with morphological sensillum types and subtypes, (2) several OBPs may be co‐localized in the same sensillum, and (3) OBP localization is not restricted to olfactory sensilla. The expression of PBPRP2 in antennal epidermis sheds some light on the possible evolution of OBPs. Microsc. Res. Tech. 55:297–306, 2001. © 2001 Wiley‐Liss, Inc.