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Association of neuronal nitric oxide synthase (nNOS) with α1‐syntrophin at the sarcolemma
Author(s) -
MiyagoeSuzuki Yuko,
Takeda Shin'Ichi
Publication year - 2001
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.1167
Subject(s) - sarcolemma , dystrophin , pdz domain , nitric oxide synthase , chemistry , microbiology and biotechnology , biology , biochemistry , myocyte , enzyme , gene
α1‐syntrophin is a PDZ‐containing dystrophin‐associated protein, expressed predominantly in striated muscle and brain. α1‐syntrophin null mice generated by gene targeting technique showed no overt muscular dystrophic phenotype. Though other dystrophin‐associated proteins were localized at the sarcolemma, neuronal nitric oxide synthase (nNOS) was selectively lost from the membrane fraction but remained in the cytoplasm. Thus, the α1‐syntrophin null mice are useful in the elucidation of the functional importance of nNOS targeting at the sarcolemma. In addition, the mice would facilitate identification of other signaling molecules, which are targeted to dystrophin complex via interaction with α1‐syntrophin. Microsc. Res. Tech. 55:164–170, 2001. © 2001 Wiley‐Liss, Inc.