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Cytochemical study of Streptococcus agalactiae and macrophage interaction
Author(s) -
Teixeira Cristina Ferreira,
Azevedo Neide Lemos,
Carvalho Técia Maria Ulisses,
Fuentes Jemima,
Nagao Prescilla Emy
Publication year - 2001
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.1137
Subject(s) - vacuole , streptococcus agalactiae , macrophage , respiratory burst , serotype , microbiology and biotechnology , acid phosphatase , phagosome , nad+ kinase , biology , nicotinamide adenine dinucleotide phosphate , nicotinamide adenine dinucleotide , oxidase test , phagocytosis , chemistry , bacteria , biochemistry , streptococcus , enzyme , in vitro , genetics , cytoplasm
Light and electron microscopy were used to analyse the process of interaction of Streptococcus agalactiae (serotypes Ia, III, and V) with resident and activated mouse peritoneal macrophages. Transmission electron microscopy showed that adherence of the S. agalactiae serotype Ia, but not III and V serotypes, to the surface of activated macrophages triggers the respiratory oxidative burst as revealed by the presence of reduced nicotinamide adenine dinucleotide (phosphate) [NAD(P)H]‐oxidase in the phagocytic vacuoles. Fusion of macrophage lysosomes with bacteria‐containing phagocytic vacuoles was observed in macrophages treated with Lucifer yellow as well as by localization of acid phosphatase for all serotypes. Microsc. Res. Tech. 54:254–259, 2001. © 2001 Wiley‐Liss, Inc.