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Differential expression of CD95, Bcl‐2, and Bax in rat gastric chief and parietal cells
Author(s) -
Neu Bruno,
Herrmuth Hedda,
Ernst Franziska,
Vaupel Wolfgang,
Reindl Wolfgang,
Hutzler Peter,
Atkinson Michael J.,
Classen Meinhard,
Schepp Wolfgang
Publication year - 2001
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.1106
Subject(s) - fas receptor , apoptosis , gastric chief cell , biology , gastric mucosa , fas ligand , enterochromaffin like cell , downregulation and upregulation , microbiology and biotechnology , foveolar cell , programmed cell death , immunostaining , parietal cell , immunology , stomach , immunohistochemistry , biochemistry , gene
Apoptotic cell death is common in the inflamed gastric mucosa, but its role in the regulation of cell homeostasis in normal gastric mucosa is unknown. We investigated the expression of CD95, Bcl‐2, and Bax and their roles in the regulation of apoptosis in normal rat gastric mucosa and in cultures of highly enriched rat chief and parietal cells by immunostaining, Western blotting, and FACS. In intact tissue CD95, Bcl‐2, and Bax were localized predominantly in the glandular base region in chief cells. In freshly isolated cells, expression of CD95, Bcl‐2, and Bax was much more pronounced in chief cells than in parietal cells. A lower intracellular Bcl‐2/Bax ratio suggesting a higher susceptibility to apoptosis was noticed in chief rather than in parietal cells. In extended cultures of parietal and chief cells, Bax expression was upregulated and Bcl‐2 expression was downregulated. These regulatory changes, presumably caused by in vitro effects, were not associated with an increase in spontaneous apoptosis. Treatment of chief and parietal cells with Fas‐ligand induced apoptosis of all CD95 expressing cells. Expression of CD95, Bcl‐2, and Bax predominantly in chief cells suggests that in this cell type regulation of apoptosis may differ from that in parietal cells. Binding of FasL with functionally active CD95 receptors on chief and parietal cells may be relevant for induction of apoptosis in inflamed gastric mucosa. Microsc. Res. Tech. 53:377–388, 2001. © 2001 Wiley‐Liss, Inc.