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Comparison of different drying procedures for scanning electron microscopy using human leukocytes
Author(s) -
TingBeall H. Ping,
Zhelev Doncho V.,
Hochmuth Robert M.
Publication year - 1995
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.1070320409
Subject(s) - scanning electron microscope , tetramethylsilane , volume (thermodynamics) , materials science , shrinkage , biomedical engineering , microscopy , flat surface , chromatography , chemistry , composite material , pathology , medicine , physics , organic chemistry , quantum mechanics
Using human leukocytes as test specimens, three different drying procedures for scanning electron microscopy: critical‐point drying (CPD), Peldri II, and tetramethylsilane (TMS), were compared. All three procedures produced identical surface morphology preservation. An equal amount of volume shrinkage was observed regardless of the dehydrants and drying techniques employed. Considering the simplicity, convenience, and time saved, air‐drying with TMS is by far the best choice for preparing animal cells for scanning electron microscopy. © 1995 Wiley‐Liss, Inc.