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Quantitative junctional permeability measurements using the confocal microscope
Author(s) -
Miller Lan
Publication year - 1995
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.1070310508
Subject(s) - confocal , confocal microscopy , microscope , epithelium , lens (geology) , embryonic stem cell , microscopy , cell , biophysics , permeability (electromagnetism) , photobleaching , fluorescence microscope , microbiology and biotechnology , chemistry , biology , fluorescence , optics , membrane , biochemistry , paleontology , physics , gene , genetics
This paper describes the use of a photobleach method and confocal microscopy to compare the cell‐to‐cell transfer rate of 5,6 carboxyfluorescein in dissociated embryonic chick lens cells with those in the anterior epithelium of the whole embryonic chick lens. The average cell‐to‐cell transfer rates obtained were 7.9 × 10 −3 sec −1 in the dissociated cells and 2.6 × 10 −3 sec −1 in the anterior epithelium in an intact lens. © 1995 Wiley‐Liss, Inc.