Premium
Section directed cryosectioning of specimens for scanning electron microscopy: A new method to study cardiac development
Author(s) -
Seo JeongWook,
Kim EulKyeong,
Brown Nigel A.,
Wessels Andy
Publication year - 1995
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.1070300606
Subject(s) - scanning electron microscope , frozen section procedure , electron microscope , section (typography) , immunohistochemistry , staining , biology , biomedical engineering , cross section (physics) , materials science , pathology , anatomy , optics , computer science , physics , composite material , medicine , immunology , genetics , quantum mechanics , operating system
A new method to study the developing heart was developed. Using this “section directed” cryosectioning method, appropriate fixed embryos can be trimmed optimally to obtain sectional planes that, if necessary, can be matched with histologically treated sections. As a result, the morphological information obtained with the scanning electron microscope can be compared in detail with the information on the molecular phenotypes of the subpopulations of cells as deducted from staining patterns of the sections. This method allows combination of the specific advantages of sophisticated histological techniques, such as immunohistochemistry and in situ hybridisation, with those of the scanning electron microscope. © 1995 Wiley‐Liss, Inc.