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Freeze‐fracture study of Bodo sp . (Kinetoplastida: Bodonidae)
Author(s) -
Vommaro Rossiane Claudia,
Attias Márcia,
De Souza Wanderley
Publication year - 1995
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.1070300305
Subject(s) - fracture (geology) , biology , membrane , intramembranous ossification , cryopreservation , morphology (biology) , anatomy , biophysics , microbiology and biotechnology , zoology , paleontology , biochemistry , embryo
Freeze‐fracture technique was used to analyse the structure of conventionally fixed and quickly frozen Bodo sp ., a free‐living kinetoplastid. In the former method, chemically fixed and cryopreserved cells presented a corrugated membrane pattern in the flagella and cell body surfaces. In the latter, however, replicas from quickly frozen unfixed flagellates showed membranes with a smoother aspect, allowing the observation of intramembranous particles (IMPs) on the fracture faces, hardly detectable in previously fixed samples. The IMPs were randomly distributed throughout the cell surface, except in the sparsely seen short IMP rows. © 1995 Wiley‐Liss, Inc.