Premium
Image analysis of the extracellular matrix
Author(s) -
Arsenault A. Larry,
Kohler Derek M.
Publication year - 1994
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.1070280507
Subject(s) - extracellular matrix , cartilage , rendering (computer graphics) , anatomy , articular cartilage , matrix (chemical analysis) , proteoglycan , vesicle , chemistry , biophysics , biology , biomedical engineering , microbiology and biotechnology , materials science , pathology , computer science , artificial intelligence , composite material , biochemistry , membrane , medicine , alternative medicine , osteoarthritis
The epiphyseal growth plate and articular cartilage matrices were preserved by slam freezing and freeze substitution to optimally retain the native organization for both cellular and matrix components. These specimens were stained and examined using conventional electron microscopic methods. The highly integrated, proteoglycan‐rich matrices were examined by computer image analysis using such parameters as distribution, connectivity, orientation, and a variety of morphometric analyses. Also, different aspects of electron tomography and 3D rendering of matrix vesicles and their associated mineral deposits from epiphyseal growth plates and turkey leg tendons are presented. © 1994 Wiley‐Liss, Inc.