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Comparative study of freeze‐substitution techniques for x‐ray microanalysis of biological tissue
Author(s) -
Påsgård Eva,
Lindh Ulf,
Roomans Godfried M.
Publication year - 1994
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.1070280309
Subject(s) - microanalysis , chemistry , substitution (logic) , ether , acetone , methanol , chromatography , analytical chemistry (journal) , nuclear chemistry , organic chemistry , computer science , programming language
A comparative study of some aspects of freeze‐substitution as a preparative method for X‐ray microanalysis of diffusible ions in biological specimens was carried out. Four substitution fluids were compared with each other and with cryosections. As criteria, elemental ratios as determined by X‐ray microanalysis of thin sections of mouse pancreas were used. Freeze‐substitution for 3 weeks in diethy ether or for 2 days in tetrahydrofurane gave results comparable to those obtained in cryosections. Slow warming to room temperature after freeze‐substitution in tetrahydrofurane gave slightly better results than fast warming. Freeze‐substitution in acetone resulted in noticeable loss and redistribution of ions and freeze‐substitution in methanol gave very poor results. © 1994 Wiley‐Liss, Inc.