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Combination of cobalt labelling with immunocytochemical reactions for electron microscopic investigations on frog spinal cord
Author(s) -
Nagy István,
Sik Attila,
Polgár Erika,
Petkó Mihály,
Antal Miklós
Publication year - 1994
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.1070280107
Subject(s) - labelling , immunogold labelling , cobalt , spinal cord , immunocytochemistry , staining , biology , frozen section procedure , anatomy , pathology , chemistry , ultrastructure , biochemistry , neuroscience , medicine , endocrinology , organic chemistry
Cobalt staining of primary afferents in frog spinal cord was combined with peroxidase‐antiperoxidase pre‐embedding or immunogold post‐embedding immunocytochemical labelling. Our results have shown that cobalt labelling can easily be distinguished from both of the immunoreaction end products. The protocol of cobalt labelling did not affect the immunoreactivity of structures. The morphology and synaptology of cobalt labelled and immunostained profiles in our sections were very similar to those reported in previous studies using different double labelling techniques. These results indicate that this new combined method could be used as an alternative double labelling technique in electron microscopic studies on nervous tissues. © 1994 Wiley‐Liss, Inc.