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Investigations of monolayers by fluorescence microscopy
Author(s) -
Stine Keith J.
Publication year - 1994
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.1070270510
Subject(s) - monolayer , fluorescence , microscopy , fluorescence microscope , chemistry , nanotechnology , langmuir , materials science , biophysics , optics , biology , organic chemistry , adsorption , physics
The technique of fluorescence microscopy enables imaging of coexisting domains in Langmuir monolayers which have been doped with a small amount of a fluorescent probe. It is also possible to image surface aggregates formed by labeled proteins interacting with phosholipid monolayers. The method has provided information concerning phase behavior and structure of monolayers and proven useful in the study of monolayers as model systems of membrance processes. © 1994 Wiley‐Liss, Inc.