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A novel cell culture technique for electron microscopy
Author(s) -
Wang Fen,
Ledford Lindsay B.,
Head Jonathan F.,
Elliott Robert L.
Publication year - 1993
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.1070260606
Subject(s) - monolayer , electron microscope , biophysics , membrane , organelle , capsule , embedment , electron micrographs , intracellular , microscopy , chemistry , cell , materials science , nanotechnology , biology , biochemistry , optics , composite material , physics , botany
A simplified technique for the monolayer growth of cultured cells and their in situ embedment on the inner surface of the pyramidal portion of the Beem capsule for electron microscopy has been developed. The results demonstrated that the cell monolayers grew well on the surface of the Beem capsule and could be embedded in situ. Electron micrographs showed cells in their natural state of contact with one another. The plasma membrane and intracellular organelles were well preserved. This method minimizes many difficult steps and eliminates the disruption of cells by scraping, pelleting, or enzymatic reaction to remove them. © 1993 Wiley‐Liss, Inc.