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Immunohistochemistry of a human type I pneumocyte‐associated protein in lung
Author(s) -
Singh Jagjit,
Singh Gurmukh,
Katyal Sikandar L.,
WongChong MariLou,
McCloskey Carol A.,
Gottron Shirley A.
Publication year - 1993
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.1070260503
Subject(s) - pathology , staining , lung , alveolar epithelium , immunohistochemistry , monoclonal antibody , biology , cuboidal cell , epithelium , antibody , respiratory epithelium , immunogen , microbiology and biotechnology , chemistry , immunology , medicine
A mouse monoclonal antibody to a human lung lavage protein was raised using proteins, with the potential ability to bind surfactant, as the immunogen. The proteins were isolated from cadaver lung lavage. The antibody was tested for its reactivity with lung and other organs. It reacted with type I pneumocytes and some of the nonciliated cells in the surface epithelium of distal bronchioles. Staining was also seen in the cells surrounding the glandular structures, superficial keratinocytes of the skin, endothelium, and nerve sheath cells. With the exception of bronchiolar cells, the stained cells have a squamous morphology, and this protein may serve as a marker or determinant of this characteristic of cells. In pathologic lungs some of the cells in air spaces with “bronchiolarization” of the epithelium exhibited staining for the protein. It could not be ascertained whether the stained cuboidal cells were reactive type II pneumocytes or distal bronchiolar cells. The intraalveolar material in pulmonary alveolar proteinosis did not show remarkable staining for the protein. Even though the protein is not unique to type I pneumocytes, it may serve as a marker for these cells in the study of their development and biology. © 1993 Wiley‐Liss, Inc.