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A modified autowasher device for rapidly washing large numbers of EM‐grids
Author(s) -
Chen Ming H.
Publication year - 1993
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.1070260212
Subject(s) - stain , grid , grid system , siphon (mollusc) , computer science , materials science , mathematics , staining , anatomy , geometry , biology , genetics
This device consists of a siphon system and 5 to 10 grid disks, modified from the previous mode (Chen, 1973), for large numbers of grids with ultrathin sections. This method improves the ease of assembling grids onto the grid disk and also requires much less stain solution. This system only takes 5 min for one single stain washing, at a maximum of 100 grids, and also avoids stain contamination. The grid disk can also be used for immunocytochemistry work and for critical point drying of grids with biological specimens.