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Inner architecture of human and simian immunodeficiency viruses
Author(s) -
Fukui Takeshi,
Imura Shunro,
Goto Toshiyuki,
Nakai Masuyo
Publication year - 1993
Publication title -
microscopy research and technique
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.536
H-Index - 118
eISSN - 1097-0029
pISSN - 1059-910X
DOI - 10.1002/jemt.1070250410
Subject(s) - simian immunodeficiency virus , simian , virology , human immunodeficiency virus (hiv) , immunodeficiency , biology , architecture , immunology , virus , immune system , history , archaeology
The cores of human and simian immunodeficiency viruses (HIV and SIV) were observed by negative staining after isolation of the core with Nonidet P40 and glutaraldehyde. Four kinds of cores were found: asymmetric and symmetric sectoral shapes, a bar shape, and a triangular shape. These results were confirmed by the examination of ultrathin sections of whole virions. In some virions, the connection between the core and the envelope was observed after freeze fracturing. Its structure was considered to be characteristic of an intermediate stage of viral maturation. The HIV‐1 core was reacted with anti‐HIV‐1 p24 mouse monoclonal antibody. © 1993 Wiley‐Liss, Inc.